2014 Fiscal Year Final Research Report
In vivo analysis of gene mutations related to epigenetic alterations in acute myeloid leukemia
| Project/Area Number |
24591411
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ANDO Kiyoshi 東海大学, 医学部, 教授 (70176014)
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| Co-Investigator(Renkei-kenkyūsha) |
YAHATA Takashi 東海大学, 医学部, 講師 (10398753)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 急性骨髄性白血病 / epigenetics / 遺伝子変異 / ヒトCD34陽性細胞 |
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| Outline of Final Research Achievements |
This study aimed to see the in vivo function of genetic mutations that are related to the epigenetic alterations in acute myeloid leukemia (AML). We focused on the DNMT3A R882H mutation among them. Human CD34+ cells transduced with DNMT3A R882H reduced the expression of myelomonocytic markers such as CD14 and the formation of CFU-mix and CFU-GM in the colony formation assays. However, these cells could not develop AML in NOG mice when they were transplanted. Taken together, these findings suggest that the transduction of DNMT3A R882H was not enough for the development of AML in vivo.
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| Free Research Field |
医歯薬学
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