Identification and functional analysis of ATL cancer stem cells using a humanized mice

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591414
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: HAMAGUCHI Isao 国立感染症研究所, その他部局等, その他 (90348780)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: HTLV-1感染モデル / ヒト化マウス / ATL幹細胞

Outline of Final Research Achievements

At the first step, we developed humanized mice by using NOJ (NOD/SCID/JAK3 KO) mouse. After CD34 positive hematopoietic stem cells were transplanted into a neonatal liver of NOJ mice, in the course of 10 weeks 30-70% human CD45 cells had been revealed. At the point of 16 weeks, enough amount of CD4 positive T cells existed. For the HTLV-1 infection, MMC treated MT-2 cells (2.5x106) were inoculated into the humanized mouse peritoneum. HTLV-1 infected T cells were increased in the mouse liver, spleen, bone marrow. These data suggested that there might be a ATL stem cell niche in the spleen. Moreover we analyzed the efficacy of new drug candidate of anti-HTLV-1 infection by using with this mouse model. This drug led to the suppression of proviral road of HTLV-1 in the peripheral blood and of infected T cell growth in the mouse model. These data suggested that the mouse model would be an effective tool for the new drug candidate.

Free Research Field

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