Development of novel differentiation induction system of hematopoietic stem cells from human induced pluripotent stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591415
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: KITAJIMA Kenji 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (10346132)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: iPS細胞 / 造血幹細胞 / ホメオボックス / 細胞分化

Outline of Final Research Achievements

We previously demonstrated that hematopoietic stem cell (HSC)-like cells were obtained from mouse induced pluripotent stem cells (iPSCs). When mouse iPSCs are differentiated into hemogenic endothelial cells (HECs) and a transcription factor Lhx2, known to induce ex vivo amplification of adult HSCs, is introduced, HSC-like cells are differentiated. Based on this finding, we challenged to induced HSCs from human iPSCs. First, we established an efficient in vitro hematopoietic differentiation induction system from human iPSCs. We found that a GSK3beta inhibitor greatly improved hematopoietic differentiation from human iPSCs. Using this method, we carried out enforced expression of Lhx2 into human iPSC-derived HECs. However, HSC-like cells were hardly obtained.

Free Research Field

幹細胞生物学