2014 Fiscal Year Final Research Report
Analysis of regulatory molecule in platelets for thrombosis employing CMK cell system
| Project/Area Number |
24591422
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KASHIWAGI Hirokazu 大阪大学, 大学院医学系研究科, 講師 (10432535)
TODOKORO Seiji 大阪大学, 大学院医学系研究科, 助教 (80403062)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | インテグリンαIIbβ3 / CMK / inside-out signaling / talin-1 / kindlin-3 |
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| Outline of Final Research Achievements |
Platelet integrin αIIbβ3 plays a crucial role in thrombosis as well as hemostasis. In this study, we have investigated the regulatory mechanism for αIIbβ3 function employing novel CMK cell system.
We demonstrated that agonist stimulation, talin-1, and kindlin-3 were crucial for αIIbβ3 activation in CMK cells: overexpression of talin head domain is sufficient for the activation of αIIbβ3 exogenously expressed on CHO cells, but not for endogenous αIIbβ3 expressed on CMK cells in the absence of agonists.
In addition, employing several talin mutants, we strongly suggested that interaction between talin-1 and membrane proximal region of β3 plays a crucila role in αIIbβ3 activation.
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| Free Research Field |
血栓・止血
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