Analyses of gene expressions related with rheumatoid arthritis and epithelial mesenchymal transition in fibroblast-like synoviocyte by adipogenesis.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591448
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Hiroshima University
• Principal Investigator: SUGIYAMA EIJI 広島大学, 大学病院, 教授 (70179167)
• Co-Investigator(Kenkyū-buntansha): YAMASAKI Satoshi 広島大学, 大学病院, 病院助教 (30367388)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 関節リウマチ / 滑膜細胞 / 脂肪分化 / 間葉系幹細胞 / 上皮間葉移行 / サイトカイン / プロテアーゼ / PPAR

Outline of Final Research Achievements

We comprehensively analyzed the gene expression in synoviocytes before and after adipogenesis. As a result, we found that MMP-1 and MMP-2 transcripts are down-regulated in synoviocyte after adipogenesis induction. The protein expressions of MMP-1 and MMP-2 are also determined. We expected that mesenchymal phenotype is diminished in synoviocyte by adipogenesis induction. As we expected, the mRNA and protein expressions of smooth muscle actin, one of mesenchymal markers, are reduced by adipogenesis induction. We found Artepillin-C, a natural PPAR ligand derived from propolis is a potent adipogenesis inducer. Consistent with the previous data, MMP-1 and MMP-2 are reduced in synoviocyte after adipogenesis that is induced by Artepillin-C. These findings suggested that adipogenesis induction of synoviocytes by using Artepillin-C contribute to the treatment of rheumatoid arthritis.

Free Research Field

リウマチ・膠原病学、骨免疫学