EBV induced erosive arthritis in humanized NOG mouse

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591457
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Nihon University
• Principal Investigator: TAKEI Masami 日本大学, 医学部, 教授 (30179600)
• Co-Investigator(Kenkyū-buntansha): SHIRAIWA Hidetaka 日本大学, 医学部, 助教 (20451338)
• Co-Investigator(Renkei-kenkyūsha): KITAMURA Noboru 日本大学, 医学部, 准教授 (40349999) ; IWATA Mitsuhiko 日本大学, 医学部, 兼任講師 (50193751) ; IKUMI Natsumi 日本大学, 医学部, 専修医 (50727857)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 関節リウマチ / EBウイルス / びらん性関節炎 / 破骨細胞 / NOGマウス / ヒト化マウス

Outline of Final Research Achievements

NOG mice are injected with human CD34+ stem cells from cord blood (LONZA) intravenously. Erosive arthritis was observed histologically at the high rate of almost 100%. Human cathepsin K stain and TRAP of multinuclear cells that exist in bone erosion were positive. Multinucleated giant cells resemble osteoclasts are observed in cultured bone marrow cells with human RANKL and M-CSF. Human cathepsin K, mitochondria stain and TRAP were all positive in these multinucleated cells.

Free Research Field

膠原病リウマチ学