Effect of anti-Mullerian hormone on germ cells of maturing rat testis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591504
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: University of Yamanashi
• Principal Investigator: KENJI Ohyama 山梨大学, 総合研究部, 医学研究員 (80051861)
• Co-Investigator(Renkei-kenkyūsha): OHTA Masanori 山梨大学, 総合研究部, 医学研究員 (80233146) ; SANO Tomoaki 山梨大学, 総合研究部, 医学研究員 (80447705) ; YAGASAKI Hideaki 山梨大学, 総合研究部, 助教 (00377540)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 抗ミュラー管ホルモン / 生殖細胞 / 精巣 / 精子形成

Outline of Final Research Achievements

The expression of AMH and AMHR2 mRNA and protein was determined to clarify the effects of of AMH on germ cells. <Methods> Animal:SD rats, Germ cell isolation:collagenase digestion and percoll gradient, Quantitative real-time PCR:postnatal 5day-35day testes, In situ hybridization(Amh, Amhr2 mRNA):21D and 49D testes, Immunohistochemistry (AMH, AMHR2 antibody):10-49D testes. <Results> In situ hybridization of 21D testis, expressions of Amh and Amhr2 were observed in spermatocytes, but negative on spermatogonia. Immunohistochemical stainings of AMH and AMHR2 in 21D were observed in spermatocytes and Sertoli cells, but not spermatogonia. The staining of AMH in 49D was positive in spermatocytes and round spermatids. The staning of AMHR2 was positive in spermatocytes, round spermatids and elongated spermatids. These results indicate that AMH expressed in developing germ cells is crucial molecule to lead precise spermatogenesis by binding to AMHR2 in spermatocyte and spermatids.

Free Research Field

小児内分泌学