p115RhoGEF promote differentiation of human acute leukemic cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 24591540
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: The University of Tokyo
• Principal Investigator: Suzuki Nobuchika 東京大学, 先端科学技術研究センター, 助教 (90247007)
• Co-Investigator(Kenkyū-buntansha): Kawamura Takeshi 東京大学, アイソトープ総合センター, 准教授 (70306835) ; Kozasa Tohru 東京大学, 先端科学技術研究センター, 特任教授 (70202059)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 細胞分化 / RH-RhoGEF / 核膜

Outline of Final Research Achievements

Here we demonstrate that p115RhoGEF, a guanine nucleotide exchange factor for Rho, directly interacts with a protein at the nuclear periphery to organize the nuclear envelope and transcriptional regulators and thus promote differentiation of human leukaemic HL60 cells. Translocation of p115RhoGEF from the cytoplasm to the nucleus, in response to all-trans retinoic acid is initiated upon its phosphorylation on the specific phosphorylation site. This novel p115RhoGEF signal into the nucleus differentiates leukaemic cells, accompanied by the canonical p115RhoGEF-RhoA-actin organization at the plasma membrane.

Free Research Field

生化学