2014 Fiscal Year Final Research Report
Interaction of arachidonic acid metabolites and bioactive substances in formation of Th2 environment
| Project/Area Number |
24591553
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | Th2 / arachidonic acid / IL-33 / TRPA1 |
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| Outline of Final Research Achievements |
We investigated an interaction between various bioactive substances and humen bronchial and skin epithelial cells. We found that (1) human bronchial epithelial cells constantly express TRPA1 and TRPV1 which are enhanced by poly IC stimulation,and(2) allyl-isothiocynanate, an agonist of TRPA1, stimulates production of IL-6 through pre-treatment of cells with poly IC. We also found that (3)the production of IL-33 is enhanced by TNFa and INFr in keratinocytes, human skin epithelial cells, and (4)anti-IL-33 antibody completely inhibits allergen induced-dermatitis in mice. These results indicate that TRPA1 is associated with airway inflammation induced by viral upper respiratory infection. Keratinocyte produced-IL-33 plays an important role in allergic inflammation in human skin diseases and IL-33 probably an important target for treatment and management of allergic diseases.
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| Free Research Field |
小児科学
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