New strategy of the therapy that applied antigenic-shift of the influenza virus.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591590
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kurume University
• Principal Investigator: KASHIWAGI takahito 久留米大学, 医学部, 講師 (70320158)
• Co-Investigator(Kenkyū-buntansha): WATANABE Hiroshi 久留米大学, 医学部, 教授 (90295080) ; HARA Koyu 久留米大学, 医学部, 准教授 (40309753)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: インフルエンザウイルス / RNAポリメラーゼ / 遺伝子複製 / 抗ウイルス薬 / PAサブユニット / PB2サブユニット

Outline of Final Research Achievements

Antigenic shift is well known as an emerging mechanism of influenza virus. Previous study showed that the antigenic shift sometimes affects as a negative factor for the viral replication. Therefore, we focused on the PA and PB2 subunits, which are essential subunits for the viral replication. Consequently, the viral replication was significantly impaired by introducing either PA or PB2 subunit as inhibitor. By fragmentation of the subunit, the inhibitory effect was emphasized. In addition we constructed the mass expression system and purification method for the fragments. Future applications are expected.

Free Research Field

ウイルス学