2014 Fiscal Year Final Research Report
Research on the molecular basis for virulence expression in mumps virus
Project/Area Number |
24591592
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
|
Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
KIDOKORO MINORU 国立感染症研究所, ウイルス第三部第三室, 室長 (00370958)
|
Research Collaborator |
KATOH Hiroshi 国立感染症研究所, ウイルス第三部第三室, 研究員 (80711712)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | ムンプスウイルス / 中枢神経病原性 |
Outline of Final Research Achievements |
Through genetic analyses of the revertant mumps virus (MuV), we revealed that the main cause of the reversion is the mutation in the V/P gene. Meanwhile,introducing the mutation of the L gene of the highly virulent strain has enhanced the neurovirulence of an attenuated MuV. In addition, the MuV isolates from vaccinal horizontal transmission cases habor the characteristic mutations in their L genes. And their neurovirulence were enhanced comparing with the original vaccine viruses. In order to assess the involvement of L gene in MuV neurovirulence, we constructed recombinant MuV (rOdate/miR) which has the L gene harboring the complement sequences of the miRNA expressed specifically in central nerve system. As the result, rOdate/miR exhibited little neurovirulence. These results strongly suggested that the main causative genes for MuV neurovirulence are V/P and L genes.
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Free Research Field |
ウイルス学
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