Development of detection of EBV infection and monitoring of immunosuppression by molecular analysis after pediatric liver trasplantation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591593
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: FUKUDA Akinari 独立行政法人国立成育医療研究センター, その他部局等, その他 (60455417)
• Co-Investigator(Kenkyū-buntansha): IMADOME Ken-ichi 国立成育医療研究センター, 母児感染研究部, 室長 (70392488) ; UMEZAWA Akihiro 国立成育医療研究センター, 生殖・細胞医療研究部, 部長 (70213486) ; KASAHARA Mureo 国立成育医療研究センター・臓器移植センター, センター長 (30324651) ; SAKAMOTO Seisuke 国立成育医療研究センター・臓器移植センター (00378689)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: EBウィルス / 低侵襲検査法 / 免疫抑制療法 / 小児肝移植 / 細胞障害性T細胞活性

Outline of Final Research Achievements

The immune function assay is a measure of cell-mediated immunity based on the peripheral CD4+ T cell adenosine triphosphate (ATP) activity. The aim of this study was to assess the correlations between the CD4+ T cell ATP activity assay and the clinical status in pediatric LDLT recipients. The recipient’s status was classified as follows: stable, infection or rejection.The CD4+ T cell ATP activity in the pediatric LDLT recipients with a clinically stable status had a lower immune response range (median 162 [interquartile range: IQR, 85-297] ATPng/ml).the correlations between the CD4+ T cell ATP activity and the status of rejection or EBV viremia were limited. We developed the method of isolation of EBV from saliva.

Free Research Field

移植外科