The exhaustive search of foreign DNAs as the pathogenic factor of angiosarcoma.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591631
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: University of the Ryukyus
• Principal Investigator: TAKAHASHI Kenzo 琉球大学, 医学(系)研究科(研究院), 准教授 (80291425)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 血管肉腫 / 発癌ウイルス / トランスクリプトーム解析 / ヘルペスウイルス

Outline of Final Research Achievements

We planned to look for the gene fragment of pathogenic virus for hemangiosarcoma, which occur frequently in Okinawa prefecture. Although the gene sequences common to zootrophic herpesvirus group were chosen and used for PCR amplification with various annealing temperatures, the herpesvirus of known or a de novo was not amplified at all.
The presence of any foreign gene was not detected by the subtraction screening. Only the gene sequences of the organism those were considered to have no intervention in pathogenesis, such as a demodex, normal cutaneous inhabitant, or candida were found to be transcribed, even though RNA was directly extracted from angiosarcoma tissue and their transcriptomes were determined exhaustively.
Thus, we could not come to determine the existence of the foreign microbes those might lead to the onset of hemangiosarcoma.

Free Research Field

分子生物学、皮膚病態学、ウイルス発癌、ゲノム解析