Establishment for ceramide imaging method by mass spectrometry imaging

2015 Fiscal Year Final Research Report

• Project/Area Number: 24591632
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Nihon University (2015); Tokyo Metropolitan University (2012-2014)
• Principal Investigator: INOUE Naoko 日本大学, 生物資源科学部, 助教 (00509515)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 脂質 / イメージング / セラミド

Outline of Final Research Achievements

Ceramide which have a wide variety of molecular species due to their fatty acid chain length　possess an important role for skin barrier function. Mass spectrometry imaging which is able to detect and visualize molecules on tissues directly, would be available for detecting specific localization of ceramides. We used murine footpad sections for establishing an appropriate method for ceramide imaging. By optimizing several conditions for analyses, we have succeeded to detect and visualize ceramide, glucosylceramide, and ceramide-1-phosphate individually. This is the first attempt to demonstrate ceramide imaging on tissue sections by mass spectrometry imaging.

Free Research Field

分析化学