2014 Fiscal Year Final Research Report
A trial for the clarification of the precise relationship between filaggrin gene mutation and atopic dermatitis by using human pluripotent stem cell
Project/Area Number |
24591651
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Dermatology
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
IGAWA Ken 東京医科歯科大学, 医学部附属病院, 講師 (00372441)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | iPS細胞 / 人工ヌクレアーゼ / アトピー性皮膚炎 / フィラグリン / 角化細胞 |
Outline of Final Research Achievements |
A filaggrin is one of important proteins which consist skin barrier and the mutations of this gene are reported to be essential precipitating factor of atopic dermatitis.In this study, we try to knock-out filaggrin gene (FLG) in human iPS cells and keratinocytes will be differentiated from FLG-KO hiPS cells and their parental hiPS cells. Then, finally, we will get isogenic (only FLG-KO or FLG-normal) keratinocytes. In this situation, we can evaluate the precise effect of FLG mutation (KO) to keratinocytes physiology.In order to KO FLG in hiPS cells, we decided to use engineered nucleases mediated gene targeting. First, we made FLG specific transcription activator-like effector nuclease (FLG-TALENs) , but we failed. Then, we switched to use CRISPR/Cas9 system and finally we succeeded and we got several clones of hiPS cells which possessed FLG-mutations (KO).
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Free Research Field |
皮膚科
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