Behavioral and pharmacological studies for the purpose of function analysis of transcription factors in brain dopamine system

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591684
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: University of Miyazaki
• Principal Investigator: ISHIDA Yasushi 宮崎大学, 医学部, 教授 (20212897)
• Co-Investigator(Kenkyū-buntansha): TAKEDA Ryuichiro 宮崎大学, 安全衛生保健センター, 教授 (90336298) ; ABE Hiroshi 宮崎大学, 医学部, 講師 (20344848) ; NISHIMORI Toshikazu 宮崎大学, 医学部, 研究員 (20112211)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: パーキンソン病 / 神経移植 / 転写調節因子 / ドパミン / 行動薬理学

Outline of Final Research Achievements

A study was carried out to examine the effects of repeated L-DOPA treatment on the distribution of the immediate-early gene (IEG) proteins (FosB, c-Fos, and Zif268) in forebrain regions in a unilateral 6-hydroxydopamine (6-OHDA) rat model of Parkinson’s disease with or without intrastriatal grafts of fetal ventral mesencephalon. During a course of repeated L-DOPA treatment, rats with a 6-OHDA lesion developed abnormal involuntary movements. In summary, differential expression of three IEG proteins was observed in the forebrain regions during a course of repeated L-DOPA treatment of 6-OHDA-treated hemiparkinsonian rats.
The other goal of this study was to investigate the effect of intrastriatal grafts of fetal ventral mesencephalon (VM) on the withdrawal response in 6-OHDA rats. The present results suggest that the intrastriatal grafts of fetal VM may relieve pain sensation induced by mechanical stimulation in 6-OHDA rats.

Free Research Field

精神医学