2014 Fiscal Year Final Research Report
Detection of activated platelet on thrombus using fucoidan conjugated with USPIO
| Project/Area Number |
24591822
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KYOGOKU Shinsuke 順天堂大学, 放射線診断学講座, 准教授
KIMIZUKA Takao 順天堂大学, 放射線診断学講座, 准教授
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 分子イメージング / 血栓 / 活性化血小板 |
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| Outline of Final Research Achievements |
There is no established method to see the activated platelet on MRI scanner. In this decade, several researcher works on this issue, and developed several contrast agents. However, its safety and feasibility is still questionable. We developed a novel contrast material using USPIO conjugated with fucoidan, that is mucopolysacchrides extracted from sea weeds. In a present study, we made a activated thrombus model with rats. In a acute cerebral infarction model, we could see the enhancement of the region on MRI. Consequently, affinity of fucoidan and P-selectin was examined gently on aortic abdominal aneurysm model. Using an immunohistochemical stain toward P-selectin and an electron microscopy, distribution of USPIO and activated platelets were confirmed.
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| Free Research Field |
放射線診断
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