2014 Fiscal Year Final Research Report
Boron-conjugated hyaluronan nanoparticles for Tumor Targeting in Boron Neutron Capture Therapy
| Project/Area Number |
24591855
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Hiroshima International University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | BNCT / hyaluronic acid / nanoparticle / tumor / targeting |
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| Outline of Final Research Achievements |
Whave developed a novel boron delivery system for BNCT using hyaluronan conjugated with borocaptate (BSH). This BSH-bearing hyaluronan nanoparticles (B-HA-NPs) are expected to deliver boron atoms into melanoma cells by receptor mediated endocytosis for antitumor application in BNCT.Novel BSH-bearing hyaluronan nanoparticles were obtained by the self-assembly in water. The critical micellar concentration of B-HA-NPs (diameter: 90-180 nm) was determined by the fluorescence probe technique using pyrene. B-HA-NPs had high stability in the retention of 10B during storage at 4-37 ℃. All boborocaptate-loaded formulations had no cytotoxic effects. B-HA-NPs were readily bound to melanoma cells, and were internalized by receptor-mediated endocytosis. Survival of the washed cells preincubated with B-HA-NPs was lowest compared to survival of control cells that were pre-incubated with B-PEG-liposomes, B-HA-liposomes, B-FA-conjugates and BSH solution.
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| Free Research Field |
製剤学
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