Possibilities of mesothelioma treatment through miRNA expressed by high LET heavy ion beams

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591859
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: LIU CUIHUA 独立行政法人放射線医学総合研究所, 放射線防護研究センター, 主任研究員 (00512427)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 悪性中皮腫 / 高LET重粒子線 / miRNAのプロファイリング / miRNAの発現 / miRNA-mimic / miRNA-inhibitor / 細胞生存率 / 細胞増殖能

Outline of Final Research Achievements

Malignant mesothelioma cells were irradiated with X-rays or high-LET carbon ion beams. Total RNA includes miRNA was extracted and miRNA profiling was analyzed. The results showed the miRNA profiling for mesothelioma cells irradiated by high-LET carbon ion and X-rays was different. Different mesothelioma cell lines have different miRNA profiling. Comparing X-rays, the miR-144-3p in MESO-1 cells was expressed higher irradiated by high-LET carbon ions. Using the miR-144-3p mimic that was transfected into MESO-1 cells, the propagation of cells was inhibited by the miR-144-3P significantly. Also, the miR-718 mimic can inhibit growing of MESO-4 cells. Those results showed using special miRNA, suggested that it might be very important for the tailor-made medical care of mesothelioma patients.

Free Research Field

放射線生物学