2015 Fiscal Year Final Research Report
Direct target genes of miRNAs involved in lung cancer
| Project/Area Number |
24591905
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Okayama University |
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Principal Investigator |
Ouchida Mamoru 岡山大学, 医歯(薬)学総合研究科, 准教授 (80213635)
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| Co-Investigator(Kenkyū-buntansha) |
OKA Takashi 岡山大学, 大学院医歯薬学総合研究科, 助教 (50160651)
ITO Sachio 岡山大学, 大学院医歯薬学総合研究科, 助教 (30335624)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | microRNA / 標的遺伝子 / 肺癌 |
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| Outline of Final Research Achievements |
Lately, miR-17-92, which is highly expressed in malignant lung cancer cells, is considered the key miRNA for tumorigenesis. However, its direct targets remain under reported. We identified the FOXP1, TP53INP1, TNFAIP3, and TUSC2 genes as miR-19a targets, using luciferase, pull-down, and western blot assays. The four miR-19a target cDNA expression vectors suppressed cell viability, colony formation, migration, and invasion of lung cancer cells.
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| Free Research Field |
分子腫瘍学
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