Identification of new specific genes in esophageal and gastric cancer stem cells by SAGE method and analyses of the gene functions

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591944
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Hiroshima University
• Principal Investigator: HAMAI Yoichi 広島大学, 原爆放射線医科学研究所, 助教 (90423384)
• Co-Investigator(Kenkyū-buntansha): OKADA Morihito 広島大学, 原爆放射線医科学研究所, 教授 (70446045)
• Research Collaborator: HIHARA Jun 広島大学, 原爆放射線医科学研究所, 講師 (10322744) ; FURUKAWA Takaoki 広島大学, 病院, 医科診療医 (00736530) ; YAMAKITA Ichiko 広島大学, 病院, 医科診療医 (70634088)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 癌幹細胞 / 食道癌 / 胃癌 / SAGE法

Outline of Final Research Achievements

We analyzed the function of specific gene selected by serial analysis of gene expression method (SAGE) for gastric and esophageal cancer stem cell.
We firstly sorted the human gastric and esophageal cancer cell line due to markers of CD44+ or － by fluorescence-activated cell sorting (FACS), and separately collected each cells with CD44+ or CD44－. Subsequently, we exhaustively analyzed and compared the gene expressions of messenger ribonucleic acid (mRNA) derived from cells with CD44+ and CD44－ marker. Analyses of gene functions for a promising specific gene in cancer stem cell (CD44+ cell) selected by SAGE method was performed by RNAi method, cell migration, cell invasion, and thiazolyl blue tetrazolium bromide(MTT) assay.

Free Research Field

消化器外科