Chemokine signaling involved in colon cancer progression

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591975
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto University
• Principal Investigator: KAWADA Kenji 京都大学, 医学(系)研究科(研究院), 講師 (90322651)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 大腸癌 / ケモカイン / 転移

Outline of Final Research Achievements

Chemokines and their receptors play key roles in leukocyte trafficking and are also implicated in cancer metastasis. By constructing SW620 cell lines with reduced expression of CXCR3 and/or CXCR4 using microRNA, we investigated in vivo metastatic activities in a mouse rectal transplantation model. CXCR3-, CXCR4-, and CXCR3/CXCR4 double-knockdowns significantly reduced metastasis to lymph nodes, liver and lungs, compared with the control. Importantly, its suppressive effect was significantly stronger in CXCR3/CXCR4 double-knockdowns. Clinical specimens of liver metastasis showed a strong inverse correlation between levels of CCL15 and Smad4. Patients with CCL15-expressing metastases showed significantly shorter times of disease-free survival than those with CCL15-negative metastases.

Free Research Field

消化器外科