2014 Fiscal Year Final Research Report
Chemokine signaling involved in colon cancer progression
Project/Area Number |
24591975
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
|
Research Institution | Kyoto University |
Principal Investigator |
KAWADA Kenji 京都大学, 医学(系)研究科(研究院), 講師 (90322651)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | 大腸癌 / ケモカイン / 転移 |
Outline of Final Research Achievements |
Chemokines and their receptors play key roles in leukocyte trafficking and are also implicated in cancer metastasis. By constructing SW620 cell lines with reduced expression of CXCR3 and/or CXCR4 using microRNA, we investigated in vivo metastatic activities in a mouse rectal transplantation model. CXCR3-, CXCR4-, and CXCR3/CXCR4 double-knockdowns significantly reduced metastasis to lymph nodes, liver and lungs, compared with the control. Importantly, its suppressive effect was significantly stronger in CXCR3/CXCR4 double-knockdowns. Clinical specimens of liver metastasis showed a strong inverse correlation between levels of CCL15 and Smad4. Patients with CCL15-expressing metastases showed significantly shorter times of disease-free survival than those with CCL15-negative metastases.
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Free Research Field |
消化器外科
|