Regeneration of circumferential intestine with mucosal layer and alimentary absorption-function using amniotic membrane: in dog experiment

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591986
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Doshisha University
• Principal Investigator: HAGIWARA AKEO 同志社大学, 生命医科学部, 教授 (90198648)
• Co-Investigator(Renkei-kenkyūsha): 筏 義人 京都大学, 名誉教授 (00025909)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 腸管再生 / 動物実験 / 再生医学

Outline of Final Research Achievements

There have been　two reasons for the difficulty to regenerate circumferential intestine, namely (1) (2). In the present study liver cells were disseminated instead of intestinal mucosal cells on the inner layer of the scaffold as the epithelial cells, and
P(LA/CL) co-polymer mesh was used as the scaffold instead of the amniotic membrane. The P(LA/CL) co-polymer mesh where the hepatic cells had been disseminated and the greater omentum were made into a double layered sheet, then the double layered sheet was wrapped overlapping three times into a shape of a tube in the abdominal cavity. Thus the tube was composed of the P(LA/CL) co-polymer mesh layer and the omentum layer overlapping three times each other. The tube was incubated in the abdominal cavity so that the epithelial cells should grow and the fibroblasts and bold capillaries should infiltrate into the tube should finally form the intestinal wall-like from.

Free Research Field

消化器外科学