Establishment of hepatocytes differentiation from human multipotent stem cells and investigation for the effectiveness of cell transplantation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591999
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto University
• Principal Investigator: YASUCHIKA Kentaro 京都大学, 医学(系)研究科(研究院), 講師 (00378895)
• Co-Investigator(Kenkyū-buntansha): UEMOTO Shinji 京都大学, 医学(系)研究科(研究院), 教授 (40252449) ; ISHII Takamichi 京都大学, 医学(系)研究科(研究院), 助教 (70456789)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 再生医学 / 間葉系細胞 / 細胞移植

Outline of Final Research Achievements

Human hepatocytes which derived from human MSCs in vitro as previously reported were transplanted into X-SCID rat via portal vein. The transplanted human hepatocytes were incorporated into rat liver and produce albumin and CYP3A4, which represented functonal hepatocytes. In addition, several transplanted hepatocytes showed PCNA activity, which represented the cell progression. Furthermore, the DPPIV expression assay revealed the histological contiguity of transplanted human hepatocytes with rat liver tissue, which representrd the physiological incorporation of transplanted cells. On the otherhand, hepatocytes transplantation into mesentery revealed the effectiveness of hepatocyte transplantation for the rescue of acute lethal liver damage occured by 90% hepatectomy. Transplanted hepatocytes work not only as compensation for hepatic function but also protection of remnant liver tissue, which identified by histological findings and ATP status in remnant liver.

Free Research Field

肝臓外科学