Evaluation of light scattering and absorption properties of in vivo rat liver using a single-reflectance-fiber probe during pre-ischemia and ischemia-reperfusion

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592016
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: National Defense Medical College
• Principal Investigator: YAMAMOTO Junji 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, その他部局等, 教授 (40342654)
• Co-Investigator(Kenkyū-buntansha): SATO Shunichi 防衛医科大学校(防衛医学研究センター), 准教授 (90502906) ; KAWAUCHI Satoko 防衛医科大学校(防衛医学研究センター), 講師 (20506505) ; HATSUSE Kazuo 防衛医科大学校(その他の部局等), 准教授 (40082284)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 虚血－再灌流 / 肝臓 / 光計測 / 拡散減衰係数 / 近赤外光 / 拡散減衰係数

Outline of Final Research Achievements

To evaluate usefulness of diffuse reflectance spectroscopy for characterization of viability/microstructure of tissues, we evaluated us’(reduced scattering coefficient), ua (absorption coefficient), StO2 (tissue oxygen saturation), and the reduction of heme aa3 in CcO (cytochrome c oxidase) of in vivo liver tissue using a single-reflectance-fiber probe with two source-collector geometries. We performed in vivo recordings of diffuse reflectance spectra for exposed rat liver of the ischemia-reperfusion model. Significant increases in ua(605)/ua(620) during ischemia indicate the reduction of heme aa3, representing a sign of mitochondrial energy failure. The change in us’ at NIR wavelength region during ischemia might show morphological changes in the cellular and subcellular structures induced by the ischemia. The results of the present study indicate the potential application of the current DRS system to evaluating the pathophysiological conditions of in vivo liver tissue.

Free Research Field

肝臓外科