Establishment of a therapy for type 1 diabetes by islet cell transplantation with mesenchymal stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592027
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kobe University
• Principal Investigator: ASARI SADAKI 神戸大学, 医学部附属病院, 助教 (20580682)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Ippei 近畿大学, 医学部, 准教授 (30379408) ; GOTO Tadahiro 神戸大学, 医学部附属病院, 特命助教 (40580684) ; FUKUMOTO Takumi 神戸大学, 医学部附属病院, 准教授 (70379402) ; KU Yonoson 神戸大学, 医学部附属病院, 教授 (40195615)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 骨髄細胞 / 間葉系幹細胞 / 膵島 / 1型糖尿病 / 細胞移植

Outline of Final Research Achievements

(1) We demonstrated that mesenchymal stem cells (MSCs) were purified by culturing the sorted C57BL/6-derived bone marrow cells (BMCs) with the PI(-)CD45(-)Ter119(-)Sca-1(+)PDGFR-α(+) markers.
(2) We showed that transplantation of C57BL/6-derived BMCs (2 x 10^7) induced stable mixed hematopoietic chimerism without GVHD in prediabetic NOD mice preconditioned with temsilorimus (5mg/kg on -day 7 and -day 1), cyclophosphamide (100mg/kg on -day 4 to -day 1) and 2Gy total body irradiation (on day 0). This immunosuppression is a novel regimen based on the clinical transplantation. We also revealed that the induced hematopoietic chimerism completely prevented insulitis and onset of autoimmune diabetes.

Free Research Field

消化器外科学