2014 Fiscal Year Final Research Report
MicroRNA expression in gallbladder cancer; effect on malignant behavior and target for the treatment
| Project/Area Number |
24592030
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHTSUKA Takao 九州大学, 大学病院, 助教 (20372766)
UEDA Junji 佐賀大学, 医学部, 准教授 (90529801)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 胆嚢癌 / miRNA / 膵胆管合流異常 / 発癌 / 癌悪性度 |
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| Outline of Final Research Achievements |
The aims of this study were to explore the expression profile of miRs and to evaluate the clinical significance of miRs expression in GBC. MiRs profile of surgically resected GBCs(n=26) and the GB with PBM(n=13) were assessed by microarray. Normal GBs(n=17) were also used as control. Up-regulated miR was subjected to quantitative reverse transcription polymerase chain reaction. The relationship between expression level of miR and clinicopathological features was analyzed. Microarray showed up-regulation of 25 miRs in GBCs. Among them, miR-155 was significantly overexpressed in GBCs than in GB with PBM(p=0.007) or normal GB(P=0.04). High expression level of miR-155 in GBCs was significantly associated with the presence of lymph node metastases(p=0.01) and poor prognosis (p=0.02). PBM did not affect regulation of miR-155. Thus, high miR-155 expression might correlate with aggressive behavior of GBCs and miR-155 would become a prognostic marker as well as a therapeutic target of GBCs.
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| Free Research Field |
医歯薬学
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