Analysis of function of long non-coding RNA associated with liver metastasis from pancreatic cancer

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kumamoto University
• Principal Investigator: NAKAHARA osamu 熊本大学, 医学部附属病院, 非常勤診療医師 (40583042)
• Co-Investigator(Kenkyū-buntansha): CHIKAMOTO Akira 熊本大学, 医学部附属病院, 講師 (10419640) ; IMAI Katsunori 熊本大学, 医学部附属病院, 非常勤診療医師 (60555746) ; HAYASHI Hiromitsu 熊本大学, 大学院生命科学研究部, 助教 (80625773) ; NITTA Hidetoshi 熊本大学, 医学部附属病院, 助教 (90555749)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 膵癌 / 転移性肝腫瘍 / HOTAIR

Outline of Final Research Achievements

1.Samples of primary lesions and liver metastasis from same patients.Pancreatic samples were retrieved from patients with unresectable pancreatic cancer at Kumamoto University Hospital.Core-needle biopsies by endoscopic ultrasound-guided fine needle aspiration(EUS-FNA)from the center of the pancreas were immediately snap-frozen in liquid nitrogen and kept at -80℃.Samples of histologically-confirmed pancreatic ductal adenocarcinoma were extracted.Total RNA was prepared by RNA kit(miRVana;、Ambion).Samples have been collected prospectively and the database has been made.We will also retrieve the RNA sample from liver metastasis of the same patients and analyze the expression of HOTAIR.Total RNA samples were obtained from 8 pancreatic cancer cell lines,and real-time RT-PCR was performed.Primers of HOTAIR have been made and then cDNA of them were constructed and analyzed.

Free Research Field

消化器外科学