Functional analysis and clinical application of the Ubiquitin - Proteasome pathway in the process of renal ischemia reperfusion injury.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592046
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Tohoku University
• Principal Investigator: MOTOYOSHI NAOTAKA 東北大学, 医学(系)研究科(研究院), 非常勤講師 (40375093)
• Co-Investigator(Renkei-kenkyūsha): SAIKI Yoshikatsu 東北大学, 大学院医学系研究科, 教授 (50372298) ; KAWAMOTO Shunsuke 東北大学, 大学院医学系研究科, 准教授 (20400244) ; ABE Takaaki 東北大学, 大学院医学系研究科, 教授 (80292209) ; NAKAYAMA Keiko 東北大学, 医学系研究科, 教授 (60294972) ; OKAMURA Masashi 東北大学, 医学系研究科, 特任助手 (60547397)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 腎虚血再灌流障害 / 腎保護 / ユビキチン・プロテアソーム系 / PPAR-γ / LXR-α / 心臓大血管外科

Outline of Final Research Achievements

We have established the porcine renal ischemia-reperfusion injury model clamping bilateral renal arteries. The pigs were allocated in the two groups; control and Bortezomib (proteasome inhibitor) administration group. We assessed the degrees of kidney damage and expression of PPAR-γ and LXR-α (PPAR-γ’s target gene) in the kidney comparing the Bortezomib administration group with control group. Serum Neutrophil gelatinase-associated lipocalin (NGAL) was used as an indicator of acute kidney injury. Histopathological changings were evaluated by the pathological scoring. Qualification and quantification of the expression of PPAR-γ, LXR-α, and downstream regulatory factors(NFκB, AP-1, NFAT and STAT-3) were evaluated by immunohistochemical staining and real-time RT-PCR method.

Free Research Field

外科系臨床医学、心臓血管外科学