Retrograde injection of plasmid DNA containing nano bubble via pulmonary vien

2015 Fiscal Year Final Research Report

• Project/Area Number: 24592084
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Niigata University
• Principal Investigator: TSUCHIDA MASANORI 新潟大学, 医歯学系, 教授 (60293221)
• Co-Investigator(Kenkyū-buntansha): KOIKE TERUMOTO 新潟大学, 医歯学系, 講師 (90635723)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 遺伝子導入 / プラスミドベクター / 肺静脈 / 逆行性投与 / ナノバブル

Outline of Final Research Achievements

Lung-targeted gene transfer is expected to revolutionize the treatment of respiratory diseases. In this study, we demonstrated that naked plasmid deoxyribonucleic acid (DNA) can be transferred into alveolar cells in normal rats, by retrograde injection into the pulmonary vein with the left pulmonary artery clamped. When a lusiferase expression plasmid vector pCAGGS-lusiferase in a reporter assay was examined, the maximal lusiferase expression was obtained when the DNA solution was injected within 30 s in a volume of 1.0 mL. We detected lacZ expression exclusively in the type II pnumocyte attached to alveolar walls by immunoelectron microscopy. Protocol for naked plasmid DNA transfer into rat lung using this hydrodynamics-based transfection method was established. However, addition of micro babble did not enhance the efficacy of gene transfer. On the other hand, micro babble enhanced the efficacy of gene transfer via antegrade injection.

Free Research Field

胸部外科