Development of high sensitivity detection method of CTCs in lung cancer using microfluidic chip system

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: OKA Soichi 産業医科大学, 医学部, 助教 (20596655)
• Co-Investigator(Kenkyū-buntansha): TANAKA Fumihiro 産業医科大学, 医学部, 教授 (10283673) ; URAMOTO Hidetaka 産業医科大学, 医学部, 准教授 (90389445) ; SO Tomoko 産業医科大学, 医学部, 講師 (00331529) ; ONAGA Takashi 富山工業技術センター中央研究所, 主幹研究員 (10416133)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 肺癌 / バイオマーカー / 循環腫瘍細胞

Outline of Final Research Achievements

Prognosis of primary lung cancer patients is not satisfactory, which is mostly caused by frequent development of distant metastasis. Although Circulating Tumor Cell (CTC) is useful for a surrogate marker of distant micro-metastasis, the CellSearch which is the only machine for CTC detection for clinical use, is insufficient sensitivity. Therefore, we developed a new microfluidic chip system of domestic, to improve sensitivity, and evaluated its basic performance. Newly developed CTC-chip not only EpCAM that have been targeted up to now showed good capture ability for other cell surface antigens. In addition, it was possible genetic analysis of the captured cells.

Free Research Field

呼吸器外科学