metabolome analysis of cerebral ischemia: exploration of biomarkers by comprehensive analysis of metabolic changes before and after cerebral ischemia

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592126
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Kobe University
• Principal Investigator: HOSODA Kohkichi 神戸大学, 医学(系)研究科(研究院), 准教授 (90403261)
• Co-Investigator(Kenkyū-buntansha): KOYAMA Junji 神戸大学, 大学院医学研究科, 研究員 (00594508) ; KOHMURA Eiji 神戸大学, 大学院医学研究科, 教授 (30225388) ; UCHIHASHI Yoshito 神戸大学, 大学院医学研究科, 研究員 (80605999)
• Co-Investigator(Renkei-kenkyūsha): YOSHIDA Masaru 神戸大学, 大学院医学研究科, 准教授 (00419475)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: metabolomics / cerebral ischemia / metabolic pathway

Outline of Final Research Achievements

We created cerebral infarction by use of rat middle cerebral artery occlusion and sampled the cerebral cortex, which was analyzed by metabolome analysis with the use of gas-chromatography/mass-spectrometry. The result implicated that the tricarboxylic acid cycle (TCA) cycle was stopped and that the anaplerotic responses such as malate-aspartate shuttle and purine nucleotide cycle were upregulated and that the pentose phosphate pathway (PPP) was also upregulated. Consequently, during ultra-acute stage of cerebral ischemia, it is most likely that TCA cycle was stopped due to ATP depletion and the anaplerotic responses was upregulated and PPP was upregulated to increase NADPH to deal with the reactive oxygen species.

Free Research Field

脳神経外科学