Role of sonic hedgehog signaling in migration of malignant glioma

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592167
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Kagoshima University
• Principal Investigator: UCHIDA Hiroyuki 鹿児島大学, 医学部・歯学部附属病院, 特任助教 (80404482)
• Co-Investigator(Kenkyū-buntansha): HIRANO Hirofumi 鹿児島大学, 医学部・歯学部附属病院, 講師 (00264416) ; YUNOUE Shunji 鹿児島大学, 医歯(薬)学総合研究科, 客員研究員 (20404478) ; ARITA Kazunori 鹿児島大学, 医歯(薬)学総合研究科, 教授 (90212646)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Glioma / Cancer stem cell / CD133 / migration / Sonic hedgehog

Outline of Final Research Achievements

This study was intended to establishment of the glioma stem cell line and evaluate the influence of SHH pathway to migration of the glioma stem cells. CD133-positive cells in malignant glioma tissue were extracted using an automagnetic cell separator and tumor spheres were obtained. Gene expression of SHH pathway was detected in almost cases of malignant glioma. It was suggested that SHH participated in maintenance of stemness and migration. In adherent cells confirmed in a process of subculture, CD133-positive cells existed more than in normal malignant glioma cell lines.

Free Research Field

脳神経外科