Intra-thecal Noggin Injection For Chronic Constriction Injury partially Ameliorates Neuropathic Pain State In the Rat.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592186
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Chiba University
• Principal Investigator: MURATA ATSUSHI 千葉大学, 医学部附属病院, 准教授 (20344997)
• Co-Investigator(Kenkyū-buntansha): YAMAZAKI Masashi 筑波大学, 医学医療系, 教授 (50281712)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: neuropathic pain / CCI / mRNA / microarray / Noggin / Alzet pump / rats / astrocytes

Outline of Final Research Achievements

We find that Noggin mRNA is down-regulated in the lumber enlargement two weeks after Chronic Constriction injury (CCI) in the rats by a Microarray and RT-PCR study. In the present study, we injected Noggin protein intrathecally using Alzet-mini pump for CCI model and see the neuropathic pain state using von Frey test for five weeks. Two weeks after CCI, Alzet-mini pump (14 days) with Noggin was inserted intrathecally. Rats whose Noggin concentration was 2microg/ml showed significantly less hyper-sensation compared with control.
We next compared intrathecal Noggin injection (2microg/ml) with BMP4 (2microg/ml), or BMP4 (4microg/ml) +Noggin (4microg/ml) injection, and compared statistically with control. Noggin injection only showed statistically significant less hyper-sensation than others. Fluorescent immunohistochemistry showed Noggin injection partially decreased astrocytes accumulation in the dorsal horn compared with PBS control one week after intrathecal Noggin injection.

Free Research Field

神経科学