Therapeutic strategy based on angiogenesis induce by microRNA in orthopaedic field

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592234
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Hiroshima University
• Principal Investigator: NAKASA Tomoyuki 広島大学, 大学病院, 病院助教 (60467769)
• Co-Investigator(Kenkyū-buntansha): OCHI Mitsuo 広島大学, 大学院医歯薬保健学研究院, 教授 (70177244) ; MIYAKI Shigeru 広島大学, 病院, 講師 (10392490) ; KAMEI Naosuke 広島大学, 病院, 病院助教 (70444685)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: microRNA / 血管新生 / エクソソーム

Outline of Final Research Achievements

The therapeutic effect of microRNA (miRNA) which play a role in angiogenesis on injury in orthpaedic field. Especially, we focused on secreted miRNAs from cell. Peripheral blood mononucleated cells were isolated from healthy subjects, and cultured. Stimuli by hypoxia environment was given during culture, and the medium with or without hypoxia were analyzed using miRNA microarray. Several miRNAs showed specific expression pattern, and over expression of these miRNAs in fibroblast induced cell proliferation. In vivo, administration of miRNA related to angiogenesis into rat model of meniscus, tendon injury and atrophic non-union showed successful results.

Free Research Field

四肢機能再建学