Study of bone graft by using a liquid-nitrogen in attempt to reconstruction in malignant bone tumors of the lower extremities

2016 Fiscal Year Final Research Report

• Project/Area Number: 24592240
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Nara Medical University
• Principal Investigator: Tohma Yasuaki 奈良県立医科大学, 医学部, 研究員 (60448777)
• Project Period (FY): 2012-04-01 – 2017-03-31
• Keywords: 移植・再生医療 / 再生医学 / 骨移植 / 骨形成

Outline of Final Research Achievements

As a surgical method of reconstruction following resecting large musculoskeletal tumors while considering the preservation of limbs, autogenous bone treated is selected. However, not only tumor cells but intact cells are killed in the treated bone. Therefore, the treated bone loses osteogenic ability. The aim of this study was to develop treated bone with osteogenic capability. We gained autologous bone marrow-derived mesenchymal cells (BMSCs) by using tissue-engineered technique and transferred these BMSCs to autologous bone treated with liquid nitrogen in an animal experimental model. The BMSCs-seeded treated bones were ectopically transplanted into subcutaneous rat sites. After that, their osteogenic capabilities were evaluated by biochemical and histological evaluations. These results indicated the suitability of tissue-engineered BMSCs to heighten osteogenic response. The autograft bone treated with liquid nitrogen was given osteogenic capability by using tissue-engineered BMSCs.

Free Research Field

四肢機能再建学