Search for novel osteoclast regulatory factors produced by bone marrow mesenchymal stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592263
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: FUJITA Hirofumi 岡山大学, 医歯(薬)学総合研究科, 助教 (20423288)
• Co-Investigator(Kenkyū-buntansha): OHMOTO Naoko 岡山大学, 大学院医歯薬学総合研究科, 非常勤研究員 (70598565) ; KOBUCHI Hirotsugu 岡山大学, 大学院医歯薬学総合研究科, 講師 (10304297)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 破骨細胞 / 骨髄間葉系幹細胞 / 炎症性サイトカイン / ハイスループットスクリーング / 質量分析

Outline of Final Research Achievements

We searched for the novel osteoclast regulatory factor produced by human bone marrow mesenchymal stem cell(hMSC) on inflammatory cytokine-induced osteoclast differentiation. Our findings showed that osteoclast differentiation by TNF-alpha was suppressed by conditioned medium of hMSC and we identified some candidate molecules of osteoclast regulatory factor using high-throughput screening and mass spectrometry.

Free Research Field

骨代謝学、細胞組織学