2014 Fiscal Year Final Research Report
The brain anandmide and genaral anesthesia
| Project/Area Number |
24592307
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | アナンダミド / 全身麻酔 / 亜酸化窒素 / カンナビノイド受容体 / リモナバン / テイルフリックテスト / RT-PCR |
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| Outline of Final Research Achievements |
The analgesic effects of nitrous oxide(N2O) were investigated using tail flick test in Wistar rats received cannabinoid 1(CB1) receptor antagonist, rimonabant. In the rats injected rimonabant intrathecally, the percent of maximal possible effect(%MPE) did not increase with N2O inhalation. However, in the group administered rimonabant intraperitoneally and intraventricularly, %MPE did not decrease from the peak level. The anandamide might interact with N2O in spinal level.
RT-PCR assays demonstrated that CB1 receptor expression in rat reduced after 2 hours of exposure of N2O. CB1 receptor is known to have decreased its expression when exposed to a ligand for a long time: desensitization. It is suggested that N2O itself indicate the cannabinoids-like action or reinforce the action of endogenous cannabinoids.
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| Free Research Field |
麻酔・蘇生学
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