Practical use with control of aquaporin for brain edema decrease using knockdown technique

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592310
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Aichi Medical University (2014); Nagoya City University (2012-2013)
• Principal Investigator: FUJITA Yoshihito 愛知医科大学, 医学部, 教授 (90238593)
• Co-Investigator(Kenkyū-buntansha): SOBUE Kazuya 名古屋市立大学大学院, 医学研究科, 教授 (90264738) ; ASAI Kiyofumi 名古屋市立大学大学院, 医学研究科, 教授 (70212462)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: aquaporin / knockdown / hypothermia

Outline of Final Research Achievements

Using vector expressing shRNA, we constructed lentivirus expressing sh RNAiof aquaporin(AQP) 4. We confirmed AQP4 knockdown that was stronger than our earlier study. We constructed hAQP4-M23 and hAQP4-M1 vector and transfected into C6 cells. We confirmed the overexpression of hAQP4-M1 and hAQP4-M23 in C6 cells. Moreover, we constructed permanent cell line of hAQP4-M23-overexpressed-cells of C6.

Free Research Field

麻酔科学