The impact of HIF-1 activation on respiratory epithelium during hypoxic insults.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592322
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: The Tazuke Kofukai
• Principal Investigator: ADACHI Takehiko 公益財団法人田附興風会, 医学研究所 第9研究部, 部長 (90252428)
• Co-Investigator(Kenkyū-buntansha): HIROTA Kiichi 関西医科大学, 医学部, 准教授 (00283606)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 低酸素誘導性因子1 / 無気肺 / タイトジャンクション

Outline of Final Research Achievements

The respiratory epithelium forms an important barrier against inhaled　pollutants and microorganisms, and its barrier function is often　compromised during hypoxic insults. Epithelial activation of hypoxia-inducible factor-1 (HIF-1) represents one feature of airway inflammation,　but the functional　importance of HIF-1 within the respiratory epithelium is　largely unknown. We　evaluated the impact of HIF-1 activation on loss of epithelial barrier function during hypoxic insults. Involvement of HIF-1 in these protective effects was confirmed using the pharmacological inhibitor YC-1 and the activator of HIF-1 DFX adopting A549 cells derived from alveolar epithelium. Expression of mRNA and protein of an subunit of Namiloride-blockable ENaC, APQ5 and molecules involving in cell-cell tight junction ZO-1, occudin, claudin were investigated. The critical involvement of HIF-1 was demonstrated.

Free Research Field

麻酔科学