2014 Fiscal Year Final Research Report
Analgesic mechanism of tetrabiopterin and cholinergic metabolism inhibitors
| Project/Area Number |
24592334
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
林 維光 , 医学部, 助教 (80242973)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | GTP cyclohydrolase 1 / dorsal root ganglion / アセチルコリン / tetrahydrobiopterin / 後根神経節 / 痛覚伝達 |
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| Outline of Final Research Achievements |
GCH1 is the first and rate-limiting enzyme in the biosynthetic pathway of tetrahydrobiopterin (BH4), an important cofactor for the synthesis of several modulator in pain neurotransmission. Recently, the importance of GCH1 as pain marker has been recognized. The activity of GCH1 is regulated by its regulatory protein GTP cyclohydrolase regulatory protein (GFRP). Here we studied the regulation mechanism of GCH1/GFRP complexation using immunohistochemistry, proximity ligation assay and blue native electrophoresis. It was observed that GCH1 and GFRP were widely expressed in population of serotoninergic, dopaminergic and nitrinergic neurons, but GCH1/GFRP complex occurs mainly in sensory system. This suggested that complex could be targeted for the develop new analgesic strategy.
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| Free Research Field |
麻酔・蘇生学
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