2014 Fiscal Year Final Research Report
An investigation on the role of HIF-1 in the regulation of microglia activity
| Project/Area Number |
24592336
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Kansai Medical University (2013-2014)
Kyoto University (2012) |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIROTA Kiichi 関西医科大学, 医学部, 准教授 (00283606)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | マイクログリア / 低酸素誘導性因子1 / 神経因性疼痛 / 酸素代謝 / 自然免疫 |
|---|
| Outline of Final Research Achievements |
Microglia is a component equivalent to macrophage serving a critical innate immunity in the central nervous system (CNS). We demonstrate that in response to activation of the microglia cell line BV-2 cells, expression of both HIF-1a and HIF-1b; increases leading to enhanced expression of HIF-1 target genes. Moreover, we also demonstrated that spinal nerve ligation (SNL) in rats dysregulated the spinal oxygen homeostasis and that spinal hypoxia induced HIF-1 activation resulting in increase of mRNA expression of ATP receptors such P2X4 and P2Y7. Our results strongly suggest that increased activation of HIF-1 occurs in the CNS in response to spinal root ligation and that HIF-1 may play a critical role in the establishment of allodynia induced by SNL in which microglia activation is essential.
|
| Free Research Field |
麻酔科学
|
