A study of the perioperative glycemic control method using pharmacological activity of GLP-1

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592340
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Kochi University
• Principal Investigator: KAWANO Takashi 高知大学, 教育研究部・医療学系, 講師 (40380076)
• Co-Investigator(Kenkyū-buntansha): YOKOYAMA Masataka 高知大学, 教育研究部医療学系, 教授 (20158380)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: グルカゴン様ペプチド-1 / インスリン / 全身麻酔薬 / 血糖管理 / 周術期

Outline of Final Research Achievements

Glucagon-like peptide-1 (GLP-1) is known to increase insulin secretion and play a crucial role in maintaining glucose homeostasis. In this study, we performed both in vivo and in vitro experiments to evaluate the interaction between GLP-1 and general anesthetics in rats. Our findings can be summarized as follows: 1) Inhalation of isoflurane led to a decrease in the basal levels of GLP-1. 2) Isoflurane attenuated the glucose-evoked increase in the levels of GLP-1 and insulin and increased the plasma glucose levels. 3) During oral glucose tolerance test, dexmedetomidine inhibited glucose-induced insulin secretion in dose-dependent manner. 3) In perifusion experiments, isoflurane inhibited glucose-induced insulin release, whereas exogenous GLP-1 enhanced it. 4) Whole-cell patches showed that GLP-1 could restore the glucose-stimulated depolarization suppressed by isoflurane. 5) Propofol has no effect on the GLP-1 release from intestinal L cells.

Free Research Field

周術期管理学