2014 Fiscal Year Final Research Report
Expression of the oncoprotein gankyrin and phosphorylated retinoblastoma protein in human testis and testicular germ cell tumor
| Project/Area Number |
24592376
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | gankyrin / testicular cancer |
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| Outline of Final Research Achievements |
We evaluated the expression of gankyrin, Rb and phosphorylated Rb (pRb) in human testis and testicular germ cell tumors by immunohistochemistry. The effects of suppression of gankyrin on phosphorylation status of Rb and cell proliferation were analyzed in vitro. Suppression of gankyrin led to suppression of Rb and cell proliferation in NEC8 cells. In testicular germ cell tumors, high expressions of gankyrin and pRb were observed in seminoma and embryonal carcinoma, whereas the expressions of both proteins were weak in histological subtypes of non-seminoma. Growing teratoma and testicular malignant transformation tissues expressed pRb strongly, but gankyrin faintly. Gankyrin is dominantly expressed in normal spermatocytes and seminoma/embryonal carcinoma, and its expression correlates well with pRb expression except in the growing teratoma and testicular malignant transformation cases. These data provide new insights into the molecular mechanisms of pathogenesis of testicular tumor.
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| Free Research Field |
testicular cancer
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