The role of pCAM, papillary renal cell carcinoma-related factor for developing novel molecular pathologic classification and differentiation-inducing therapy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592396
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Kochi University
• Principal Investigator: KARASHIMA Takashi 高知大学, 教育研究部医療学系, 講師 (60304672)
• Co-Investigator(Kenkyū-buntansha): SHUIN Taro 高知大学, 教育研究部医療学系, 教授 (70128601) ; KAMADA Masayuki 高知大学, 教育研究部医療学系, 講師 (90304683)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 腎癌 / 細胞接着因子 / 乳頭状腎癌

Outline of Final Research Achievements

Papillary cell adhesion molecule (pCAM) is a adhesion molecule of cell-cell and cell-basal lamina and expressing in only neuroendocrine tissue. We examined whether the pCAM was a key molecule of cell differentiation and pathological architecture in papillary renal cell carcinoma (RCC). A human RCC cell line, 786-O highly expressing pCAM was undifferentiated clear cell RCC. Downregulation of the pCAM expression made the 786-O tumor to be well differentiated papillary RCC in the kidney of athymic nude mouse. Re-expression of pCAM by site-directed mutagenesis method returned 786-O tumor to be undifferentiated clear cell RCC. We demonstrated the architectural transformation accompanied with changing epithelial mesenchymal transition (EMT) factors by PCR array 786O. In conclusions, the pCAM is a key molecule for papillary formation of RCC.

Free Research Field

医歯薬学