2014 Fiscal Year Final Research Report
Exploration of novel biomarkers for prostate cancer by proteomic and pathway analysis
| Project/Area Number |
24592411
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 前立腺癌 |
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| Outline of Final Research Achievements |
In the present study, shotgun nano LC-MS/MS proteomics using laser-microdissection (LMD) of formalin-fixed and paraffin-embedded (FFPE) tissues was conducted to identify grade- and stage-related biomarkers of prostate cancer (PCa).
Nano LC-MS/MS proteomics was conducted using FFPE tissue of PCa. The tissue samples included specimens with low Gleason score (GS), specimens with high GS, and biopsy specimens from metastatic PCa. Targeted cells were microdissected for proteomic analysis. Candidate proteins were selected by pathway analysis and evaluated by immunohistochemistry. Immunohistochemical evaluation was attempted to validate candidate markers. The expression of MCCC2 was significantly higher in cancerous tissue than in benign prostatic tissue. Nano LC-MS/MS proteomics using LMD of FFPE specimens was useful for exploring biomarkers in solid tumors, including PCa. We found potential biomarker candidates with expression profiles related to grade and stage of PCa.
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| Free Research Field |
前立腺癌
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