2014 Fiscal Year Final Research Report
Bone marrow-derived cell sheets restore structure and function of radiation-injured urinary bladders
| Project/Area Number |
24592427
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 膀胱再生 / 排尿障害 / 放射線照射傷害 / 骨髄細胞 / 温度応答性培養皿 / 細胞シート / ラット / Tissue Engineering |
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| Outline of Final Research Achievements |
The urinary bladder is one of the organs most often affected by the side effects of pelvic radiotherapy. In this study, we produced bone marrow-derived cell sheets in temperature-responsive culture dishes that release the monolayer sheets intact. We then determined if the recovered cell sheets could restore function to irradiated urinary bladders. After 4 weeks, the cell sheet-transplanted bladders had smooth muscle layers and nerve fibers in proportions that were significantly larger than those of the control bladders. In cystometric investigations, cell-free sheet control rats had frequent and irregular periods of micturition; however, the cell sheet-transplanted rats voided at regular intervals of micturition, which is typical for rats with uninjured bladders. There were 24 growth factors to promote reconstruction of urinary bladders in the cell sheet-transplanted urinary bladders. In conclusion, cell sheet engineering has great potential to restore damaged urinary bladders.
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| Free Research Field |
泌尿器科学
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