Functional urotherial-sheet reconstruction from transformed epithelial cells using hollow-fiber cell-culture system

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592435
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: MARUYAMA Tetsuji 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (50305546)
• Co-Investigator(Kenkyū-buntansha): MIZUNO Kentaro 名古屋市立大学, 大学院医学研究科, 講師 (70448710) ; HAYASHI Yutaro 名古屋市立大学, 大学院医学研究科, 准教授 (40238134) ; KOHRI Kenjiro 名古屋市立大学, その他部局, 学長 (30122047) ; KOJIMA Yosiyuki 福島県立医科大学, 医学部, 教授 (60305539)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: KIT陽性細胞 / シェアーストレス / 伸展刺激 / ホローファイバー / 上皮間葉系誘導 / 平滑筋細胞 / 尿路上皮細胞 / 再生医療

Outline of Final Research Achievements

With cytoengineering method, we have made urotherial sheet with functional smooth muscle layer showing regular special pattern. These cells were under laminar flow stimulation exerted shear and stretch stress, and/or electrical stimulation using hollow-fiber cell-culture system. In addition, ES cells were used to induce these urotherial and smooth muscle cell to minimize preparing time and effort.

Free Research Field

医歯薬学　外科系臨床医学・泌尿器科学