Analysis of the role of cyclooxygenase 2 on spermatogenesis disturbance.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592449
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: KUBOTA Hiroki 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (10347403)
• Co-Investigator(Kenkyū-buntansha): KOHRI Kenjiro 名古屋市立大学, その他の部局, 学長 (30122047) ; HAYASHI Yutaro 名古屋市立大学, 大学院医学研究科, 准教授 (40238134) ; SASAKI Shoichi 名古屋市立大学, 大学院医学研究科, 准教授 (50225869) ; UMEMOTO Yukihiro 名古屋市立大学, 大学院医学研究科, 講師 (80381812)
• Research Collaborator: PARRINGTON John Oxford大学
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Cyclooxygenase2 / Spermatogenesis

Outline of Final Research Achievements

In this study, we examined whether COX-2 was induced in impaired testes, and also investigated the possible role of COX in the testes using experimental cryptorchidism model mice. Five-week-old male mice underwent an operation to induce unilateral cryptorchidism, and they were then divided into three groups. Immunohistological staining and RT-PCR revealed that the expression of COX-2 was increased in the experimental cryptorchid testes. TdT-mediated dUTP-biotin nick end-labeling (TUNEL) staining revealed that the number of apoptotic cells was significantly increased by COX-2 inhibitor. However, the COX-1 inhibitor did not appear to affect spermatogenesis in the experimental cryptorchid testes. These results suggest that the COX-2 inhibitor provoked testicular damage in experimental cryptorchidism by inducing germ cell apoptosis. The expression of COX-2 might be induced to protect germ cells from heat stress caused by experimental cryptorchidism.

Free Research Field

アンドロロジー