Analysis of lysophospholipid mediators in human follicle growth toward good-quality oocytes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592468
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Nagoya University
• Principal Investigator: IWASE Akira 名古屋大学, 医学部附属病院, 病院教授 (20362246)
• Co-Investigator(Kenkyū-buntansha): GOTO Maki 名古屋大学, 医学部附属病院, 病院講師 (90378125)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Tomoko 名古屋大学, 医学部附属病院, 病院助教 (40732681) ; NAKAHARA Tatsuo 名古屋大学, 医学部附属病院, 助教 (50534830)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 卵胞発育 / 顆粒膜細胞 / 脂質メディエーター / コレステロール合成 / S1P / GDF-9

Outline of Final Research Achievements

An oocyte-derived growth factor, growth differentiation factor-9 upregulated the cholesterol-synthetic enzymes in human granulosa cells. We found the significant differences in the expression of the cholesterol-synthetic enzymes between luteinized granulosa cells derived from pregnant and non-pregnant follicles.
A lysophospholipid mediator, shingosine-1-phosphate (S1P) activated the PI3K-Akt pathway and stimulated proliferation of human granulosa cells. In addition, S1P inhibited H2O2-induced apoptosis of granulosa cells.

Free Research Field

産婦人科学